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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
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Image Search Results


The gluD homologues from closely related bacterial species were expressed in the C . difficile gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and ELISA ( E & H ).

Journal: PLoS ONE

Article Title: Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo

doi: 10.1371/journal.pone.0160107

Figure Lengend Snippet: The gluD homologues from closely related bacterial species were expressed in the C . difficile gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and ELISA ( E & H ).

Article Snippet: The presence of C . difficile GDH in the cecal contents was detected with a commercially available ELISA kit (CDiff Check TM- 60, TechLab Inc., Blacksburg, Va.) in accordance with the manufacturer's instructions.

Techniques: Mutagenesis, Expressing, Construct, SDS Page, Staining, Enzyme-linked Immunosorbent Assay